This is the second volume covering the papers presented at this biennial conference. As with the last volume, a topic has been chosen and speakers invited to talk with specific reference to this particular growth area. The focus of this volume is on the analysis of peptides and proteins. With the development of new ionization methods and expanded capabilities for the analysis of high mass compounds, mass spectrometry is becoming an essential and more widely used method in the arsenal of techniques used to characterize the primary structure of peptides and proteins. The topics covered in this book include instrumentation developments, quantitation, enzyme kinetics, sequence analysis by instrumental/enzymatic approaches and applications in the pharmaceutical, medical and research laboratories. The current problems in protein research and the role of mass spectrometry in relation to other methods of protein analysis are also included.
Plasma desorption mass analysis in immunochemistry, G.Lindenberg et al; the oxime-based segment synthesis of Cro., J.W.Kelly et al; the use of 252Cf plasma desorption spectrometry for the analysis of synthetic peptides and proteins, B.T.Chait; plasma desorption mass spectrometric analysis of glycopeptides, I.Jardine; optimization and use of nitrocellulose as a matrix for peptide and protein desorption mass spectrometry, P.Roepstorff et al; fast atom bombardment mass spectrometry as a probe of the specificity and kinetics of enzymic reactions, R.M.Caprioli and W.T.Moore Jr; structural characterization of biopharmaceuticals by MS and tandem MS, S.A.Carr et al; the limited proteolysis of polypeptides, T.Keough et al; the structure determination of peptides by tandem mass spectrometry, S.A.Martin et al; protein sequence analysis by tandem mass spectrometry - new methods and instrumentation, D.F.Hunt et al; peptide and protein sequencing by thermospray LC-MS, K.Stachowiak et al; deuterium exchange studies for structural confirmation of small peptides using a thermospray mass spectrometer interface, M.M.Siegel; thermospray LC/MS for the analysis of peptides - 1,000-4,000u, P.J.Rudewicz; isolation and sequencing of peptides from the middle molecular weight fraction of uremic filtrate, U.Rohr et al; combined thin layer chromatography/252Cf-plasma desorption mass spectrometry for drug monitoring, H.Jungclas et al; aggregation, glycosylation and glutathione - peptide behaviour on and off the foil, L.Silly et al; picomole analysis of peptides and proteins by liquid secondary ion mass spectrometry, B.W.Gibson et al; 252Cf plasma desorption mass spectrometry of peptides and proteins, P.D.Mudgett et al; improving sensitivity of 252-Cf-PDMS for peptides/proteins, R.D.Macfarlane et al; recent developments in laser desorption and laser ionization mass spectrometry of peptides and proteins, J.Grotemeyer; time-of-flight measurements of daughter ions from small peptides, K.G.Standing et al; recent developments in high mass ionization and detection systems for magnetic sector mass spectrometers, A.E.Ashcroft et al; improving the detection sensitivity for high mass ions, J.F.Mahoney et al; detection probability and secondary electron emission from microchannel plate surfaces by peptide molecular ions, P.W.Geno and R.D.Macfarlane; entropic processes in heavy ion induced desorption of biomolecules applied to peptides, E.R.Hilf and H.F.Kammer.