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Full Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.
Contents
Genome mining and sequence analysis of chemosensory soluble proteins in arthropods
Joel Vizueta, Paula Escuer, Alejandro Sánchez-Gracia and Julio Rozas
Reconstructing protein coding sequences from ancient DNA
Michael Hofreiter and Stefanie Hartmann
Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs
Jean-François Picimbon
Proteomics of arthropod soluble olfactory proteins
Francesca R. Dani and Giuseppe Pieraccini
Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects
Andrea Scaloni
Bacterial expression and purification of vertebrate odorant-binding proteins
Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loïc Briand
Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography
Anaïs Gaubert, Béatrice Amigues, Silvia Spinelli and Christian Cambillau
Solution structure of insect CSP and OBPs by NMR
Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone
Stability of OBPs
Nadja Hellmann
Ligand-binding assays with OBPs and CSPs
Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi
Fine structure immunocytochemistry—An important tool for research on odorant-binding proteins
Rudolf Alexander Steinbrecht
Effect of OBPs on the response of olfactory receptors
Bing Wang, Song Cao, Wei Liu and Guirong Wang
Site-directed mutagenesis of odorant-binding proteins
Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi
Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing
Yusuke Shiota and Takeshi Sakurai
Electrochemical biosensing with odorant binding proteins
Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu
Electronic biosensors based on graphene FETs
Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll
Electronic biosensors based on EGOFETs
Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli
Gravimetric biosensors
Khasim Cali, Elena Tuccori and Krishna C. Persaud
Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins
Anil Bozdogan, Simone Hageneder and Jakub Dostalek
